新型PH-20疫苗不同注射方式对雌性豚鼠免疫应答和避孕效果的影响

Immune response and effect of contraception in female guinea pigs injected with PH-20 coupling body

目的观察以等比方法配制的新型精子表面特异性透明质酸酶(PH-20)疫苗的不同注射方式对豚鼠免疫应答和避孕的影响。方法 60只雌性豚鼠随机分为两大组(直肠注射组和腹腔注射组),每组再分为3个小组(实验1组、实验2组和对照组,每小组各10只),用电镜观察该新型PH-20疫苗的形态,应用双夹心酶联免疫吸附试验(ELISA)检测豚鼠阴道分泌物中分泌型免疫球蛋白A(sIgA)的水平、放射免疫分析法检测血液sIgA的水平、竞争ELISA技术检测血液PH-20特异性抗体的水平。结果直肠注射组和腹腔注射组豚鼠血液和阴道分泌物中sIgA、PH-20抗体含量均较对照组升高(P均<0.05);实验1组(添加弗氏完全佐剂)豚鼠在各测量时间点血液和阴道分泌物中sIgA、PH-20抗体含量均较实验2组(未添加弗氏完全佐剂)高(P均<0.05)。直肠注射组(50%)和腹腔注射组(76.7%)总阳性怀孕率相比有显著性差异(P<0.05)。结论该新型PH-20疫苗具有较高的免疫活性,能诱导机体产生明显的体液反应,且直肠注射诱导免疫优于腹腔注射。用弗氏完全佐剂均能增强两种注射方式的避孕效果。

Objective To observe the immune response and contraceptive effect of guinea pigs injected with the PH- 20 coupling body by different method of injection. Methods Sixty female guinea pigs were divided randomly into two groups （rectal injection group and intraperitoneal injection group ） , then each group was subdivided into three groups （ experimental group 1, experimental group 2 and control group, l0 female guinea pigs in each group）. To observe mor- phology of the coupling by electron microscopy, to test vaginal secretory immunoglobulin A （sIgA） level by double sand- wich ELISA and blood slgA by radioimmunoassay. PH-20 specific antibody level in blood was detected by competitive ELISA. Results Blood and vaginal secretions slgA and PH-20 antibody levels in rectal injection group and intraperito- neal injection group were both higher than those in control group （ P 〈 0.05）. Blood and vaginal secretions of guinea pig slgA and PH-20 antibody levels in experimental group 1 （add Freund complete adjuvant） were higher than those in ex- perimental group 2 （without adding Freund complete adjuvant） （P 〈 0.05 ）. The total positive pregnancy rate in rectal injection group was significantly different from intraperitoneal injection group （ P 〈 0.05 ）. Conclusions The PH-20 coupling body has a high immune activity and can induce significant humoral response, and the rectal injection is superior to the intraperitoneal injection. Freund＇ s complete adjuvant is able to enhance the effectiveness of two contraceptive in- jection.