牛滋养层干细胞样细胞系的建立

Isolation and Cultivation of Bovine Trophoblastic Stem-like Cell Lines

为了成功建立牛滋养层干细胞样细胞系,探讨牛滋养层干细胞样细胞(Bovine Trophoblastic Stem-like Cells)体外培养条件及相关影响因素,以胎牛子宫成纤维细胞条件培养液作为培养基,胶原铺被培养皿,利用体外受精牛囊胚培养得到牛滋养层干细胞样细胞,并调整培养体系,以探讨其最适生长条件。结果表明:利用添加FBS、β-巯基乙醇、肝素以及胎牛子宫成纤维细胞条件培养液的DMEM/F12,体外培养牛囊胚,可以初步建立牛滋养层干细胞样细胞系。

The study aimed to investigate the proper conditions and influential factors in order to best establish a trophoblastic stem-like cell line,derived from in vitro matured and fertilized blastocysts.The cells were cultured on collagen coated wells using fetal bovine fibroblast-conditioned medium with an adjustable culture system used to investigate the effect on cell growth.The author found the the DMEM/F12 medium was mixed with fetal bovine fibroblast-conditioned medium at a dilution of 1：1 and supplemented with FBS,heparin and β-mercaptoethanol was the best culture medium.