铜离子污染胁迫对蚯蚓重要抗氧化物酶活性的影响

Effect of Copper Ion on Activities of Important Antioxidant Enzymes from Earthworms

为了探究铜离子(Cu2+)对土壤生态环境中重要的生物蚯蚓的毒性作用,笔者研究了人工土壤中硫酸铜污染胁迫对安德爱胜蚓(Eisenia andrei)的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性的影响。结果表明,低浓度的Cu2+对蚯蚓CAT酶有一定的激活作用,且酶活随着染毒时间,呈现先降低后升高的变化。随着Cu2+在蚯蚓体内的富集,对SOD呈现短暂抑制后,明显激活的作用。Cu2+会引起蚯蚓体内GSH-PX活性变化,但并不规律。3种抗氧化物酶活性变化程度与土壤中给予的Cu2+浓度没有剂量依赖性。

In order to study the toxicity of copper ion to earthworms in the soils,the author reported the effects of copper ion （Cu 2 ＋） on activities of catalase （CAT）,superoxide dismutase （SOD） and glutathione peroxidase （GSH-PX） from earthworms.The results indicated that CAT was activated partially by Cu 2＋ in the lower dose.The activity was decreased at first,then that was activated with time.SOD was decreased then was activated significantly.GSH-PX was fluctuation with the increasing of Cu 2＋ in the earthworm,but it was not shown the order all the exposure time.The activities of CAT,SOD and GSH-PX were not relative to the exposure doses of copper in the artificial soils.