摘要
甘蔗是异源多倍体植物,染色体数目多、体积小,染色体制片困难,导致细胞遗传学研究进展缓慢。以分裂旺盛的甘蔗根尖为材料,对不同采样时间、预处理方法、解离时间、染色方法等各技术环节进行实验研究。结果表明:在冬季气温较低的条件下,于15:00进行采样,饱和对二氯苯与0.002mol/L8-羟基喹啉等体积混合液室温预处理4~4.5h,1mol/LHCl与45%乙酸等体积混合液于60℃水浴锅解离8min后,用改良苯酚卡宝品红进行涂片滴染,所获得的染色体制片效果最好。
Sugarcane belongs to the allopolyploid plant which contains large number of chromosome with small size.The cytological research on sugarcane may hence be influenced by its difficulties to obtain chromosome products successful.In this paper,the root-tips of sugarcane were used to study the effects of the sampling time,the pretreatment chemical solution,hydrolysing time and staining on the chromosome squashing.The results showed that the optimal sampling time of roots was 15:00 pm under the lower temperature condition in winter.Among the four pretreatment of chemical solutions,the solution mixed with equal volume of saturated p-dichlorobenzene and 0.002 mol/L 8-hydroxyquinoline,was pretreated at room temperature for 4-4.5 h got best result.Root tips were then hydrolysed with the solution mixed by equal volume of 1 mol/L HCl and 45% acetic acid,for 8 min at 60℃,and stained with modified Carbol fuchsin solution.With this method a very good effect of chromosome squashing could be obtained.
出处
《中国农学通报》
CSCD
北大核心
2011年第27期104-108,共5页
Chinese Agricultural Science Bulletin
基金
国家人事部留学人员科技活动优秀项目"甘蔗与蔗茅属远缘杂交的分子细胞遗传学研究"
云南省应用基础研究计划重点项目"甘蔗核心种质构建与评价"(2006C0013Z)
云南省中青年学术技术带头人后备人才培养项目"甘蔗种质资源鉴定评价与创新利用"(2009CI059)
农业部作物种质资源保种项目"甘蔗种质资源保护与利用"(NB2011-2130135-18)
关键词
甘蔗
根尖
染色体
制片技术
sugarcane
root-tips
chromosome
mounting technique