摘要
目的:建立注射用黄芩苷原料有关物质的检查方法。方法:采用高效液相色谱法,色谱柱为ODS C18柱(250mm×4.6mm,5μm),流动相为甲醇-水-磷酸(47:53:0.2),检测波长为280nm,柱温为35℃。结果:6批注射用黄芩苷原料有关物质含量均低于2.5%。结论:所建立的方法简便、灵敏、准确,可用于注射用黄芩苷原料有关物质的检查。
Objective:To establish the inspection method of related substances of raw material for injection baicalin.Methods:HPLC,the ODS C18 column(250mm×4.6mm,5μm)was used,a mixture of methanol-water-phosphoric acid(47:53:0.2)was used as the mobile phase and the detection wavelength was 280nm,the column temperature was 35 ℃.Results:Six batches of raw material for injection baicalin content of related substances is less than 2.5%.Conclusion:The method is simple,sensitive,accurate,and it can be used for inspection of related substances of raw materials for injection baicalin.
出处
《辽宁中医药大学学报》
CAS
2011年第12期63-65,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
辽宁省教育厅重点实验室项目(2008S149)