蔷薇属植物无菌体系的建立

Development of an in vitro culture system of Rosa spp.Plants

[目的]建立蔷薇属植物的无菌快繁体系。[方法]以茎段为外植体,以10种蔷薇属植物为试验材料,在培养基中添加不同的同激素组合来建立其组织培养体系。[结果]10种蔷薇属植物在萌发培养基MS+0.5 mg/L BA+0.01 mg/L NAA上均可萌发,芽体健壮,萌发率可达到70%以上;10种蔷薇属植物各自适宜的增殖培养基略有不同,在增殖培养过程中,增殖率可以达到3.0%以上,丛生苗健壮,叶色正常;健壮小苗在添加0.1～0.2 mg/LNAA的1/2MS培养基上诱导生根,根系生长良好,生根率可以达到90%～100%;将生根后的小苗驯化后移栽,成活率在95%左右。[结论]建立了蔷薇属植物的无菌快繁体系,为蔷薇属植物的分子育种奠定了基础。

[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish their in vitro tissue culture system with the stem segments as explants.[Result] All ten varieties tested germinated when the nodal segment explants were cultured on the sprouting medium MS＋ 0.5 mg/L BA ＋0.01 mg/L NAA and grew vigorous shoots,and the sprouting rate was up to 70%.Of the ten rose varieties tested,each has a respective optimal proliferation medium,and the multiplication rates for all the varieties reached 3.0%.The axillary buds were vigorous and normal in leaf color.The optimal medium for rooting and acclimationwas 1/2MS medium containing 0.1 mg/L or 0.2 mg/L NAA,in which the rooting frequency reached 90%-100% and the root system was developed.After acclimation and transplant,the survival rate was as higher as 95%.[Conclusion] An in vitro tissue culture system of Rosa spp.has been established in this study,which lays foundation for the molecular breeding of Rosa spp.