miR-622在胃癌细胞中的表达及其功能的研究

Expression and biological function of miR-622 in gastric cancer cells

目的研究miR-622在胃癌细胞中的表达，探讨miR－622在胃癌中的生物学功能。方法采用实时荧光定量PCR检测miR-622在胃癌细胞中的表达，以胃癌细胞SGC-7901和NCI．N87为模型瞬时转染miR-622寡核苷酸前体或抑制体，通过克隆形成、侵袭和划痕实验验证其在胃癌细胞中的功能。结果荧光定量PCR实验结果表明：miR--622在胃癌细胞SGC-7901中相对表达量为1．29±0．58，而在NCI．N87细胞中相对表达量为10．96±1．02。在SGC-7901细胞中转染了miR-622寡核苷酸前体，克隆形成率为76％。划痕试验中愈合能力为（11±7）μm，胃癌细胞侵袭能力为（732±3）个，与对照组相比差异均有统计学意义（均P〈0．05）。结论miR-622能够促进胃癌细胞克隆形成、迁移和侵袭。

Objective To investigate the biological function of miR-622 in human gastric cancer cell lines of SGC-7901 and NCI-N87 cells and its role in gastric carcinogenesis. Methods We analyzed the expression of miR-622 in those human gastric cancer cell lines by quantitative real-time polymerase chain reaction. Tumorigenesis, migration and invasion ability of miR-622 overexpression was assessed in vitro with miR-622 precursor and inhibitor in in SGC-7901 and NCI-N87 cells. Results The expression level of miR-622 in SGC 7901 was 1.29 ± 0. 57, and it was 10. 96 e 1.02 in NCI-N87 cells. The soft agar colony formation rate was 76% in SGC-7901 after transfecting miR-331-3p precursor, the ability of scratch healing was （ 11 ± 7 ） μm, and the ability of transwell invasion was （ 731 ± 3 ） , compared with that in control group, the differences were statistically significant （ P 〈 0.05 ）. Condusions Over-expression of miR-622 promotes tumorigenesis, migration, and invasion in gastric cancer cells in vitro.