DARPP-32在大鼠全脑的定位分布

Immunohistochemical localization of DARPP-32,a dopamine and cyclic-AMP-regulated phosphoprotein,in the rat brain

目的探讨DARPP-32在大鼠全脑的表达分布特点。方法应用免疫组织化学染色技术对大鼠脑内DARPP-32的表达分布进行观察。结果免疫组织化学染色结果显示,强阳性的DARPP-32染色大部分分布于基底节区和前嗅皮质区,主要分布在伏隔核、尾壳核及杏仁核复合体的神经元胞体内,以及苍白球、腹侧苍白球、脚间核及黑质网状部的神经纤维中;中～弱阳性DARPP-32免疫染色见于中脑红核、隔伞核、小脑的普肯耶神经元、内侧缰核、皮层及海马等区域;阳性DARPP-32主要定位于神经元的胞质、胞核和胞膜上。结论 DARPP-32在大鼠全脑广泛表达,但主要分布于多巴胺能神经元密集投射的脑区,这些区域的神经元富含多巴胺D1受体。DARPP-32可能在多巴胺介导的神经功能中扮演着重要角色。

Objective To study the distribution of DARPP-32 in the rat brain. Methods Immunohistoehemical staining techniques were applied to study the localization of DARPP-32 in neurons of the rat brain. Results The strong immunoreactivity for DARPP-32 was localized primarily in the basal ganglia and the anterior olfactory area, involving neuronal cell bodies or dendrites in the nucleus aecumbens, caudate putamen, amygdaloid nucleus and fibers throughout the globus pallidus, ventral pallidum and pars reticulate of the substantia nigra. The moderate to weak immunoraetivity for DARPP-32 was observed in red nucleus, septofimbrial nucleus, purkinje cells in cerebellum, medial habenula nucleus, cortex and hippocampus. DARPP-32 was present in the cytoplasm, cytomembrane and cell nucleus of labeled neuronal somata and dendrites. Conclusion DARPP-32 is widely distributed in the rat brain. It is primarily present in the neurons, which receive a dopamine input and contain dense of the D1 receptor. DARPP-32 may play an important role in dopaminoceptive neurons.