微管相关蛋白2和巢蛋白在人胚胎脊髓的表达

Expression of microtubule-associated protein 2 and nestin in the developing spinal cord of the human embryo

目的探讨微管相关蛋白2(MAP-2)和巢蛋白在人胚胎脊髓发育阶段的分布规律及其表达意义。方法应用免疫组织化学法,检测第2、3、4月龄段共16例人胚胎脊髓前角、中央管及后角中MAP-2和巢蛋白的表达、分布状况。结果第2～4个月龄段,人胚胎脊髓内均可见巢蛋白表达阳性的神经纤维分布,随着胎龄的增大,脊髓前角处巢蛋白阳性表达的神经前体细胞和神经胶质细胞呈先增多再降低的趋势,在后角呈逐渐增高趋势。MAP-2在脊髓前后角神经细胞胞质中呈阳性表达,随胎龄增大而增强。结论 MAP-2和巢蛋白参与调控人胚胎脊髓神经细胞和神经胶质细胞的生长发育。

Objective To investigate distribution of the microtubule-associated protein 2 （MAP-2） and nestin in the human developing embryonic spinal cord. Methods Sixteen human embryos, ranged from 2 to 4 months old were used in the present study. Immunohistochemical staining was uesd to detect the expression and distribution of MAP-2 and nestin in the anterior horn, central canal and posterior horn of the spinal cord. Results At the stages of the second to fourth months, nestin-positive nerve fibers were seen within the human embryonic spinal cord, with increase of the gestational age, the expression of nestin-positive neural precursor cells and glial cells first increased and then decreased in the anterior horn, but that in the posterior horn gradually increased. MAP-2 in the anterior and posterior horns of spinal cord nerve cells were positive, with increasing gestational age. Conclusion MAP-2 and nestin involve in the regulation of spinal cord neurons and glial cells during the human embryonic growth and development.