FOXP3、GITR和GITRL在口腔扁平苔藓患者外周血中的表达

Expression of FOXP3,GITR and GITRL in peripheral blood in patients with oral lichen planus

目的:研究FOXP3和GITR/GITRL基因在口腔扁平苔藓(OLP)患者外周血中的表达水平。方法:采用实时荧光定量RT-PCR的方法,检测30例OLP患者和15例健康人外周血中FOXP3、GITR和GITRL的基因表达水平。结果:OLP组FOXP3、GITR和GITRL mRNA表达量均显著低于健康对照组(P<0.01);结果经2-ΔΔCT转换后,OLP组FOXP3、GITR和GITRL mRNA表达水平分别是健康对照组的23.4%、18.1%和20.9%;FOXP3和GITR的表达水平呈正相关关系(r=0.491,P<0.05)。结论:FOXP3和GITR/GITRL mRNA的表达异常,影响了调节性T细胞(Treg)的功能。

Objective： To study the expression of FOXP3 and GITR/GITRL genes in peripheral blood of patients with oral lichen planus（OLP）.Methods： mRNA expression of FOXP3,GITR and GITRL in peripheral blood of 30 OLP patients and 15 healthy subjects was detected by real-time fluorescent quantitative RT-PCR method.Results： In patients with OLP,mRNA expression of FOXP3,GITR,and GITRL was significantly lower than in the controls（P〈0.01）.After the results were converted by the 2-ΔΔCT,the mRNA expression levels of FOXP3,GITR and GITRL in OLP were 23.4%,18.1% and 20.9% of those in the controls respectively.The expression of FOXP3 and GITR showed a positive correlation（r=0.491,P〈0.05）.Conclusion： The abnormal expression of FOXP3 and GITR/GITRL genes may influence the function of regulatory T cells（Treg） and play a role in the pathogenesis of OLP.