蛋白质对半饥饿大鼠肠黏膜形态的影响

Effect of supplementation of protein diet on morphology of intestinal tract in semi-starvation rat

目的:研究半饥饿大鼠肠黏膜形态变化与补充蛋白质对保护肠黏膜的作用。方法:将24只大鼠随机分为正常对照组、50%正常饲料组、50%饲料+蛋白组。2周杀死大鼠取小肠,观察肠黏膜形态的变化,测定小肠黏膜超氧化物歧化酶(SOD)、丙二醛(MDA)和DNA含量。结果:50%饲料+蛋白组大鼠的小肠壁厚度、绒毛高度均好于50%正常饲料组,小肠DNA含量、SOD活性明显高于50%正常饲料组(P<0.05);肠黏膜MDA含量明显低于50%正常饲料组(P<0.05)。结论:半饥饿状态下,增加大鼠饲料中蛋白质的比例,对半饥饿大鼠肠黏膜形态有一定的保护作用。

Objective： To study the effects of supplement of protein diet on morphology of intestinal mucosa in semi-starvation rats.Methods： Twenty-four male Wister rats were randomly divided into normal control,50% intake group,50% intake with supplementation of protein diet group.The morphology were observed in intestinal villus,and SOD,MDA and DNA were determined in intestinal mucosa.Results： Intestinal wall thickness and villus height in 50% intake with supplementation of protein diet group were raised compared with 50% intake group（P0.05）.The content of DNA in the 50% intake with supplementation of protein diet group were higher than that in control group（P0.05）.The activity of SOD was increased significantly and MDA level in serum in 50% intake with supplementation of protein diet group was decreased（P0.05）.Conclusion：The supplementation of protein in diet may effectively protect the morphology of intestinal mucosa in semi-starvation rats.