缺氧诱导因子1α在人胰腺导管腺癌中的表达及其与微血管形成的关系

Expression of Hypoxia Inducible Factor 1α and its Relationship to Angiogenesis in Human Pancreatic Ductal Adenocarcinoma

目的:探讨胰腺导管腺癌组织中缺氧诱导因子1α(hypoxia inducible factor 1α,HIF-1α)表达与血管内皮生长因子(vas-cular endothelial growth factor,VEGF)表达、微血管密度之间的关系及其临床意义。方法:运用免疫组织化学方法对2009年10月—2010年10月手术切除的43例胰腺导管腺癌标本和20例正常胰腺组织对照标本分别进行HIF-1α、VEGF和CD34检测,再结合肿瘤的临床和病理学特征进行相关分析。结果:HIF-1α、VEGF在胰腺癌组织中表达均显著高于正常胰腺组织(67.4%比10.0%和76.7%比35.0%,P<0.01),胰腺癌组织中的微血管密度(micro vessel density,MVD)计数也显著高于正常胰腺组织(21.95±7.83比11.95±3.90,P<0.01)。HIF-1α表达与VEGF、MVD等呈正相关,且HIF-1α表达与肿瘤的直径、临床分期以及有无淋巴结或远处转移相关,而与肿瘤的部位和病理学分级之间无关。结论:HIF-1α在人胰腺导管腺癌中高表达,并与胰腺癌微血管形成以及胰腺癌的生物学行为关系密切,有望为胰腺癌的治疗和预后判断提供参考。

Objective：To assess the expression of hypoxia inducible factor 1α （HIF-1α）, vascular endothelial cell growth factor （VEGF）, and the micro vessel density （MVD） in pancreatic ductal adenocarcinoma （PDA） and their clinical significance. Methods： The expression of HIF-1α, VEGF and CD34 was measured by immunohistochemistry in 43 pancreatic ductal adeno carcinoma and 20 normal pancreatic tissue samples. Then the relationship between HIF-1α and the clinical or pathologic charac ter of PDA was analyzed respectively. Results： The positivity of HIF 1α, VEGF and CD34 in PDA was significantly higher than in normal pancreatic tissue （67.4% vs. 10.0% and 76.7% vs. 35.0%, P〈0.01）. The MVD in PDA was also higher than in normal pancreatic tissue （21.95± 7.83 vs. 11.95 ± 3.90,P〈0.01）. The expression of HIF-1α was correlated positively with VEGF and MVD. HIF-1α expression was associated with tumor size, advanced tumor stage, lymph node metastasis and distant metastasis, but not with the localization of tumor and the grade of pathology. Conclusions： This study suggested that HIF-1α is over expressed in human pancreatic ductal adenocarcinoma and is closely associated with the neogenesis of micro blood vessel. It may play an important role in carcinogenesis or aggression in pancreatic cancer and may he a useful marker for evaluating prognosis in pancreatic cancer.