人乳头瘤病毒DNA检测与液基薄层细胞学检查在宫颈疾病筛查中的应用

Application analysis of HPV testing and thinprep cytologic test in screening of cervical lesion

目的:探讨液基薄层细胞学与高危型HPV-DNA检测在妇科宫颈疾病筛查的应用及其临床意义。方法:分析733名妇女宫颈刷出物中13种高危型HPV-DNA检测结果,其中227例同时进行TCT细胞学检查。根据细胞学诊断结果分为4组,同时对各组HPV DNA检测结果进行统计学分析。结果:733名妇女高危型HPV感染率为16.2%。227例TCT结果显示各年龄组TCT阳性率差别没有统计学意义(P=0.203)。各分组的高危型HPV检出率分别为9.5%(17/179)、63%(17/27)、90.9%(10/11)和80%(8/10),差别具有统计学意义(P=0.000),在TCT阳性的病例中(A0以上),高危型HPV DNA检出率随着细胞异常级别的升高而升高的趋势并不明显。结论:联合应用高危型HPV检测和液基薄层细胞学(TCT)检查可以提高宫颈疾病筛查阳性率。

Objective：To explore the application of human papillomavirus（HPV） detection and Thinprep Cytology test（TCT） as well as their clinical significance in screening cervical lesions.Methods： The detection results of 13 high-risk HPV-DNA（HR-HPV） from cervical scrapes of 733 women were retrospectively reviewed,in which 227 women were performed TCT and the results have also been analysed.According to the cytological results,227 women were divided into four groups and the results of HR-HPV of every group were analyzed.Results： Among 733 women,119（16.2%）were found to be high-risk HPV positive.On the whole,the infection rates of HR-HPV in all age groups had no significant difference（P=0.568）,and it was the same case with the abnormal rates of TCT in all age groups（P=0.203）.The detection rates of HR-HPV DNA in four groups were 9.5%,63%,90.9% and 80%.Overall,the infection rates of HR-HPV in the above four cytological gradings had significant difference（P=0.000）.Among the TCT positive cases（above AO）,the trend that detection rates of HR-HPV DNA increased with the rise of the abnormal grade was not obvious.Conclusion： Combination of TCT and HPV testing can improve the positive predictive value in screening of cervical epithelial lesions.