摘要
应用DNA随机扩增多态性(RAPD)分子标记技术研究了光合细菌(PSB)对Cd、Pb及呋喃丹污染土壤的微生物群落DNA序列多样性的影响。结果表明,Cd、Pb及呋喃丹单一污染或3者复合污染土壤的微生物群落DNA序列的丰富度相对对照土样(S0)都有不同程度的增加,受Cd、Pb或呋喃丹污染,可能会引起土壤微生物群落DNA序列本身发生变化;S0与加入PSB的土样(Cd、Pb及呋喃丹单一污染土样或3者复合污染土样)微生物群落间的DNA序列的相似系数要高于S0与不加PSB的土样(Cd、Pb及呋喃丹单一污染土样或3者复合污染土样)微生物群落间的DNA序列,PSB对改善土壤微生物群落DNA序列的组成有积极的影响。
The effect of photosynthetic bacteria（PSB） on DNA sequence diversity of soil microbial communities contaminated by cadmium,lead and carbofuran was evaluated by using random amplified polymorphic DNA（RAPD） fingerprints.The results showed that DNA sequence richness of single cadmium,lead,carbofuran contaminated soil and compound polluted soil was higher than that of control soil sample（S0）.Cadmium,lead and carbofuran contamination could vary the DNA sequence of microbial communities in soil.The similarity coefficients of microbial community DNA sequences between S0 and contaminated soil（cadmium,lead,carbofuran and compound polluted soil） containing PSB was higher than that between S0 and contaminated soil without PSB.PSB had positive impact on improving soil microbial communities DNA sequence.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2011年第11期24-28,共5页
Environmental Pollution & Control
基金
山西省科技攻关计划项目(No.20080311027-1)
