摘要
在广东发现了可能被番茄斑萎病毒属(Tospovirus)病毒侵染的西瓜,采用ELISA和RT-PCR法对该西瓜病样进行了检测,西瓜病叶粗汁液不与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)的血清发生反应;利用引物J13/UHP通过RT-PCR可以扩增出约1400 bp的基因片段,该片段包括一个840 bp的核衣壳蛋白ORF,其推导的氨基酸序列与已报道的Melon yellow spot virus(MYSV)NP基因氨基酸序列的同源率都为99%,进化树分析表明侵染广东西瓜的病毒(命名为MYSV-GZ)属于Tospovirus的MYSV血清组。
A watermelon sample possibly infected by tospovirus in Guangdong was detected by ELISA and RT-PCR.Crude extracts from yellow spot watermelon leaves were not react with antisera of Tomato spotted wilt virus（TSWV） and Watermelon silver mottle virus（WSMoV） by ELISA.When they were tested by RT-PCR using primer pair J13/UHP,about 1400bp fragment was amplified,which contained 840bp ORF of nucleocapsid protein（NP）.Sequence analysis showed that the NP shared 99% identity at the amino acid level with those reported NP of Melon yellow spot virus（MYSV）,phylogenetic analysis confirmed that the virus infected watermelon in Guangdong（designated as MYSV-GZ） was belonged to MYSV serogroup of Tospovirus.
出处
《植物检疫》
北大核心
2011年第6期37-39,共3页
Plant Quarantine
基金
广东省自然科学基金(07300670)
