水鼩鼱消化道5-羟色胺阳性细胞的形态与分布

The Immunohistochemical Localization and Morphology of 5-HT Positive Cells in Digestive Tract of Neomys fodiens

应用免疫酶标技术ABC(avidin-biotin-peroxidase complex)法,对水鼩鼱(Neomys fodiens)消化道内5-羟色胺(5-HT)阳性细胞的分布及形态进行研究。结果显示,5-羟色胺阳性细胞仅在肠内分布,食管和胃处没有分布。细胞分布密度曲线呈抛物线型,空肠部分布密度最高,十二指肠部次之,直肠处最低。5-HT细胞主要分布于消化道上皮细胞之间、上皮基部、腺泡上皮细胞之间。形态较单一,仅具有圆形和锥体形。根据细胞的结构形态可以认为,水鼩鼱消化道内的5-HT细胞兼具内分泌与外分泌2种功能。

Immunohistochemical localization and morphology of 5-hydroxytryptamine immunoreactive cells（5-HT cells） in the digestive tract of Neomys fodiens were investigated by using the ABC（avidin-biotin-peroxidase complex）immunostaining technique.The 5-HT positive immunoreactive cells could be observed throughout the intestinal tract,from duodenum to rectum.The cell density curve appeared parabolic,with the highest in the jejunum（4.50±1.60）,followed by the duodenum（4.00±1.33）,while the lowest in the rectum（1.60±0.94）.The 5-HT cells distributed at the bottom of epithelial cells,between epithelial cells,between glandular epithelial cells or in the lamina propria.The 5-HT cells showed two shapes,round and taper.It is suggested that 5-HT positive immunoreactive cells in the digestive tract of N.fodiens should have both endocrine and exocrine functions,and their distributive density is related to its living habit and the way of existence.