多发性骨髓瘤成骨细胞活性和Runx2的表达

Osteoblast activity and Runx2 expression in multiple myeloma

目的研究多发性骨髓瘤(MM)患者间充质干细胞(MSCs)向成骨细胞(OB)分化过程中Runx2的表达,探讨MM骨形成障碍的机制。方法以4例有明显骨质破坏的MM患者为研究对象,2例缺铁性贫血和1例特发性血小板减少性紫癜为对照,体外诱导MSCs向OB分化,采用CCK-8试剂盒检测MSCs向OB分化过程中的增殖能力,以平均光密度值(AOD)表示;碱性磷酸酶(ALP)染色和钙结节(Von-Kossa)染色检测MSCs的OB形成能力,分别以IOD sum/Area sum值和平均钙结节个数表示;逆转录-聚合酶链反应(RT-PCR)技术检测MSCs向OB分化过程中Runx2的表达,以Runx2/GAPDH平均光密度值表示。结果在MSCs向OB分化过程中,MM患者MSCs的增殖能力、ALP表达、钙结节形成能力、Runx2表达均明显低于对照组(P<0.01)。结论 MM患者骨髓MSCs向OB分化过程中的增殖能力和OB形成能力均下降,可能与Runx2的表达减低有关。

Objective To evaluate Runx2 expression of mesenchymal stem cells（MSCs） from bone marrow of multiple myeloma（MM）,and discuss the mechanism of osteoblast inhibit ion in MM.Methods Bone marrow was collected from four MM patients with obvious osteolytic lesion.The control group included two patients with iron deficiency anemia and one with idiopathic thrombocytopenic purpura.MS C s were induced to differentiate into OB in vitro.CCK-8 kit was employed to ana lyze MSCs proliferation,defined as the average optical density（AOD）.Alk aline phosphatase staining and Von-Kossa staining were performed to assess oste oblast formation,defined as IOD sum/Area sum and the average of calcium nodules respectively.RT-PCR assays were applied to detect Runx2 expression of MSCs,d e fined as Runx2/GAPDH average optical density.Results The r e was significant difference in the proliferation of MSCs between MM and control group.The osteoblast formation was less in MM group than that of control g roup.RT-PCR assays showed that Runx2 expression was significantly lower in MM than that in control group（all P0.01）.Conclusions In the course of differentiation of MSCs into OB,bone marrow MSCs fro m MM patients are characterized with a low proliferation and osteoblast formatio n,which may be associated with lower expression of Runx2.