转Bt基因稻谷对印度谷螟生长发育的影响

Effects of transgenic Bt(Cry1Ab/Cry1Ac) rice on the population development of Plodia interpunctella

为建立仓储阶段转Bt水稻安全性评价中靶标害虫抗性汰选研究体系,配制了含不同比例(70%,50%,30%,10%)转Bt基因(Cry1Ab/Cry1Abc)明辉63水稻谷粉(简称Bt谷粉)的人工饲料饲喂印度谷螟Plodia interpunctella(Hübner),测定其对1～3龄幼虫在72h内的急性毒力,及对印度谷螟种群生长发育的影响,并采用ELISA法检测转基因稻谷和末龄幼虫体内Bt蛋白含量。结果发现:4种比例人工饲料对幼虫的毒力作用均发生在取食48h后,72h后剂量效应明显。含Bt水稻较高比例的饲料对印度谷螟发育的负面效应明显:幼虫死亡率高,发育历期延长。Bt蛋白在幼虫体内含量与对应饲料中的含量基本成正比。综合考虑,将Bt杀虫蛋白含量2.35μg/g作为转Bt基因稻谷对印度谷螟的亚致死剂量最为合适。

The effect of the transgenic Bt rice Cry1Ab/Cry1Ac gene on the feeding of Plodia interpunctella（Hübner） on stored rice was investigated.Population parameters of P.interpunctella were investigated after adding different ratios（70%,50%,30%,10%） of transgenic Bt rice flour into a conventional artificial diet at 30℃ and RH75%.The content of Bt protein in 3rd instar larva and the transgenic Bt rice grain was measured using the ELISA method.The results show that significant mortality of 1st instar larva was observed 72 h after feeding on Bt rice flour.Larval mortality rates and development time increased significantly as the content of transgenic Bt rice flour in the artificial diet increased.For research purposes,2.35 μg/g was the optimal sub-lethal dose of Cry1Ab/Cry1Ac protein for P.interpunctella.