持续皮下注射碱性成纤维细胞生长因子对家兔超长随意皮瓣成活的影响

BFGF Continuous Subcutaneous Injection by Analgesia Pump to the Survival of Extra Long Free Skin Flap in Rabbits

目的探讨镇痛泵持续皮下注射BFGF对家兔超长随意皮瓣成活的影响。方法将12只家兔随机分为BFGF持续治疗组(A组)、BFGF治疗组(B组)和对照组(C组),每组4只,均构建背部随意皮瓣(6.0 cm×1.5 cm),皮下埋针。A组持续治疗3 d,B和C组第1 d2、d3、d进行BFGF治疗和0.85生理盐水注射,术后7 d分别观察皮瓣的成活面积及成活率,并行新生血管计数的检测及免疫组化检查。结果皮瓣成活率A组为75.41%±2.19%,B组为54.38%±4.52%,C组为35.43%±2.71%。皮瓣中段温度:各组皮瓣中段在术后即刻无显著差异,从第2～3 d起开始有显著差异(P<0.05),各组从第4～7 d开始则基本平稳(P<0.01)。结论持续皮下注射BFGF能够更好地提高随意皮瓣的成活率,内皮细胞增殖的激发可能有一个时间过程。

Objective To investigate the effect of BFGF continuous subcutaneous injection by analgesia pump to the survival of extra long free skin flap in rabbits.Methods 12 rabbits were randomly divided into three groups,BFGF continuous treatment group（A group）,BFGF treatment group（B group）and control group（C group）（n=4）,with the construction of free skin flap（6.0 cm×15 cm） and hypodermic needle-embedding therapy in the back of rabbits in each group.A group received continuous treatment for 3 days,while B and C group received BFGF and 0.85 saline injection on the 1st day,2nd days,and 3rd day.We observe the survival area of the skin flap,calculate the survival rate of it,detect the number of new blood vessels and the change of immunohistochemistry on the 7th day after operation.Results The survival rate of skin flap in A group is（75.41±2.19）%,B group is（54.38±4.52）%,while C group is（35.43±2.71）%.The temperature of the middle part in the skin flap of each group have no significant difference in the immediate moment after operation,while have significant difference（P0.05） in the 2nd and 3rd day after operation and have extremely significant difference（P0.01） in the 4th and 7th day after operation.Conclusion BFGF continuous subcutaneous injection will improve the survival rate of free skin flap,and mightbe the stimulation of endothelial cell proliferation need time process.