中波紫外线对人角质形成细胞MMP-9 mRNA的影响

Study on the expression of Matrix Metalloproteinase-9 Caused by Ultraviolet B Irradiated immortalized human Keratinocyte Cells

目的:研究中波紫外线(UVB)诱导的永生化人角质形成细胞中MMP-9 mRNA的表达,探讨其与UVB照射所致的细胞损伤的关系。方法:绘制细胞生长曲线,采用MTT方法测定UVB照射后细胞的增殖活性,反转录-聚合酶链反应(RT-PCR)方法测定UVB照射后HaCaT细胞中MMP-9 mRNA的表达。结果:UVB照射后,HaCaT细胞的增殖活性受到抑制,MMP-9 mRNA表达增强;随UVB剂量增加,照射组的MMP-9 mRNA表达逐渐增多,与未照光组比较,差异有统计学意义(P<0.01)。结论:UVB照射可引起HaCaT细胞中MMP-9 mRNA表达显著增加,且与UVB照射剂量呈正相关,与光老化的发生有一定的关系。

Objective To explore the expression of MMP-9 after UVB irradiation on immortalized human keratinocyte cells（HaCaT cells）.Methods Draw cell growth curve,and MTT method for determining cell proliferation activity.The HaCaT cells were irradiated by different doses of UVB and RT-PCR was used to detect the expression of MMP-9 mRNA in HaCaT cells.Results With the increase of UVB doses,the expression of MMP-9 mRNA in HaCaT cells increased（P0.01）.Conclusion UVB irradiation may induce the expression of MMP-9 mRNA in HaCaT cells which maybe have some role in the pathogenesis of skin photoaging.