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拟南芥CYP76C2基因(At2g45570)的克隆及其菌核病抗性功能分析

Cloning of Arabidopsis thaliana CYP76C2(At2g45570) and analysis of its resistance to Sclerotinia sclerotiorum
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摘要 以拟南芥基因组DNA为模板,扩增出细胞色素P450(CYP76C2)基因的ORF,成功构建了CYP76C2基因的过表达载体并获得转基因植株.通过离体和活体接种表明,CYP76C2基因的过表达植株对核盘菌的抗性增强. Cytochrome P450（CYP76C2） was amplified using Arabidopsis genomic DNA as template,over-expression vectors of CYP76C2 were successfully constructed and transgenic plants were obtained.The over-expression CYP76C2 plants showed significant resistance to Sclerotinia sclerotiorum in detached leaves and in vivo.
作者 陈晓婷 孙翠霞 王爱荣 鲁国东
机构地区 福建农林大学生命科学学院 福建农林大学功能基因组研究中心 福建农林大学植物保护学院
出处 《福建农林大学学报（自然科学版）》 CSCD 北大核心 2011年第6期606-609,共4页 Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金 国家自然科学基金项目(30671347) 公益性行业(农业)科研专项(nyhyzx07-05) 中国博士后基金面上资助项目(20100480710) 福建农林大学博士后项目(80254)
关键词 拟南芥 细胞色素P450 菌核病 草酸 Arabidopsis thaliana cytochrome P450 Sclerotinia sclerotiorum oxalic acid
分类号 S432.4 [农业科学—植物病理学]
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参考文献15

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二级参考文献31

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共引文献31

福建农林大学学报（自然科学版）
2011年 第6期

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