胰腺癌组织微血管密度及淋巴管密度的改变及临床意义

Clinical signigicances of measuredvalue of microvessel density and lymphatic vessel density in pancreatic carcinoma tissue

目的:研究胰腺癌组织中微血管密度(MVD)和淋巴管密度(LVD)的变化、与胰腺癌临床病理的联系及相互关系。方法:采用免疫组织化学SABC法应用CD34检测41例胰腺导管腺癌患者配对癌组织和正常胰腺组织中MVD的表达情况,应用D2-40检测配对癌组织、癌旁组织及正常胰腺组织中LVD的表达情况,分析MVD和LVD与肿瘤分化程度、分期和淋巴转移间的相关性以及癌组织MVD与癌旁组织LVD表达之间的关系。结果:癌组织及正常胰腺组织MVD分别为46.585±16.935和11.100±4.036,两组差异有统计学意义(P=0.000)。癌组织、癌旁及正常胰腺组织LVD分别为11.244±4.800、15.829±7.470和13.512±5.139;其中癌旁组织与正常胰腺组织LVD差异无统计学意义(P=0.060),癌旁组织与癌组织的LVD差异有统计学意义(P=0.000)。胰腺癌组织MVD与癌旁组织LVD间存在相关性(P=0.025<0.05)。结论:胰腺导管腺癌组织中MVD及LVD与肿瘤分化程度、病理分期及淋巴转移相关;胰腺癌组织内MVD与癌旁组织LVD间存在相关性。

Objective： To study the changes of the microvessel density （MVD） and Lymphatic vessel density （LVD） and the relationship with the clinical pathology parameer and their interaction. Methods： The MVD and LVD were detected by using CD34 and monoclonal antibody D2-40 in 41 patients with pancreatic ductal adenocarcinoma by using immunohistochemical method SABC. The expression of MVD and LVD in the cancer, paraneoptastic and normal pancreatic tissue were analyzed relationship with the clinical pathology parameter like as the pathological stage, tumor differentiation and lymph node metastasis, and the correlation between the MVD of cancer tissues and LVD of paraneoplastic. Result： The mean MVD value in paired cancer and normal pancreatic tissue were 46.585 ± 16.935,11,100 ± 4.036 respectively. The statistically significant difference was found between the two groups （P=0.000 〈 0.01）. The mean MVD value in paired cancer and normal pancreatic tissue were 46.585 ± 16.935,11.100 ± 4.036 respectively. The statistically significant difference was found between the two groups（P=0.000 〈 0.01 ）.The average LVD of cancer, paraneoplastic and normal pancreatic tissue were 11,244±4.800, 15.829 ±7.470 and 13.512±5.139 respectively in 41 cases, in which significant differerce was found between the paraneoplastic and cancer tissue （P=0. 000 〈 0.01） and contrary between paraneoplastic and normal pancreatic tissue （P=0.060）.Correlation was found between the MVD values of tissues and the LVD values of paraneoplastic tissues （P=0. 025 〈 0.05）. Conclusion： The MVD values and LVD values of pancreatic ductal adenocarcinoma were associated with the tumor differentiation, pathological stage and lymph node metastasis. The MVD values of pancreatic cancer tissues was correlated with the LVD values of paraneoplastic tissues.