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环介导等温扩增过程监测与结果分析技术研究进展

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摘要 2000年,Notomi等^1]一种新颖的核酸扩增方法一环介导等温扩增(100p-ediated sothermal mplification,LAMP)。该技术利用4种不同的特异性引物识别靶基因的6个特定区域,在恒温条件进行反应,不需要模板的热变性和长时间温度循环。
出处 《医学研究杂志》 2011年第11期165-168,共4页 Journal of Medical Research
基金 国家自然科学青年基金资助项目(21005086)
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参考文献18

  • 1Yamazaki W, Ishibashi M, Kawahara R, et al. Development of a loop - mediated isothermal amplification assay for sensitive and rapid de- tection of vibrio parahaemolyticus [ J ]. BMC Microbiol, 2008, 8: 163 - 170.
  • 2Mori Y, Notomi T. Loop - mediated isothermal amplification (LAMP) : a rapid, accurate, and cost - effective diagnostic method for infectious diseases [J]. J Infect Chem, 2009, 15(2) : 62 -69.
  • 3Le Roux CA, Kubo T, Grobbelaar AA, et al. Development and eval- uation of a real - time reverse transcription Loop - mediated isothermal amplification assay for rapid detection of rift valley fever virus in clini- cal specimens [ J]. Clin Microbiol, 2009, 47 (3) : 645 - 651.
  • 4夏永恒,杨兵,张杰,袁蕾磊,孙继国,周宏专,苏霞.2种鸡免疫抑制性疾病LAMP检测方法的建立[J].中国动物检疫,2009,26(5):29-32. 被引量:8
  • 5Sally L. Wastling, Kim Picozzi, Abbas S. L. Kakembo, Susan C. Welburn. LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats [ J]. PLoS Negl Trop Dis, 2010, 4( 11 ) : E865.
  • 6Xiaofeng R, Pengchong L. Development of reverse transcription loop - mediated isothermal amplification for rapid detection of porcine epi- demic diarrhea virus [J]. Virus Genes, 2011, DOI 10. 1007/s 11262-011 -0570-3.
  • 7刘永生,丁耀忠,张杰.环介导等温扩增(LAMP)技术的应用研究[J].中国农学通报,2010,26(8):87-89. 被引量:17
  • 8Sun W, Qin P, Gao H, et al. Electrochemical DNA biosensor based onehitosan/nano - V205/MWCNTs composite - modied carbon ionic liquid electrode and its application to the LAMP product of Yersinia enterocolitica gene sequence[J]. Bioelectron, 2010, 25 (6) : 1264 - 1270.
  • 9Nakamura N, Ito K, Takahashi M, et al. Detection of six single -nu- cleotide polymorphisms associated with rheumatoid arthritis by a loop -mediated isothermal amplification method and an electrochemical DNA chip [J]. AnalChem, 2007, 79(24): 9484 -9493.
  • 10刘业兵,张磊,宁宜宝,王琴,范学政.猪细小病毒LAMP检测方法的建立[J].中国农业科学,2010,43(14):3012-3018. 被引量:27

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