肾结核患者尿液抗酸杆菌与自然杀伤T细胞的关系

Relation between peripheral blood natural killer T cells and urine tubercle bacillus in patients with nephrotuberculosis

目的探索肾结核患者尿液抗酸杆菌与自然杀伤细胞T(natural killer T cells,NKT)功能的相关性。方法将54例肾结核患者分为活动期组(n=28)及非活动期组(n=26),并选取20名健康者作为对照;采用多色流式细胞仪检测外周血NKT细胞百分含量,同法检测NKT胞内细胞因子白介素-4(IL-4)、干扰素-γ(IFN-γ)及白介素-12(IL-12)含量;采用荧光定量PCR法检测尿液抗酸杆菌TB-DNA含量,使用ELISA法检测尿液细胞因子IL-4、IFN-γ及IL-12含量。结果在外周血NKT及其细胞因子、以及尿液细胞因子含量比较方面,均为活动期患者<非活动期患者<健康人;而尿液TB-DNA含量比较显示活动期患者>非活动期患者>健康人,以上任意两组间均有显著性差异(P<0.05)。相关性分析表明外周血NKT与尿液TB-DNA含量呈显著负相关(r=-0.878,P<0.000 1)。结论肾结核患者NKT细胞功能明显降低,且与是否处于活动期密切相关;反映病灶局部的尿液抗酸杆菌的含量与反映全身免疫功能的NKT细胞数量级功能均有紧密联系。

Objective To explore the correlation between peripheral blood （NKT） and the quantity of urine tubercle bacillus. Methods 56 male patients with nephrotuberculosis developed from primary pulmonary tuberculosis （PTB） were divided into the active stage group （group A, n = 28） and inactive stage group （group B, n = 26）, according to their clinical manifestations and laboratory examinations. 20 health men were chosen as controls （group C）. The quantity of NKT together with its intracellular cytokines, including IL-4, IFN-γ and IL-12 was detected with polychromatic flow cytometric resection. The quantity of TB-DNA was detected with real time PCR, and the contents of IL-4, IFN-γ and IL-12 were examined with ELISA. Results The peripheral blood NKT and the intracellular cytokines among the three groups were compared, and the sequence for the three groups was observed as： A〈B〈C, with significant difference （P〈0. 05）. Whereas the sequence of urine TBDNA in the groups was A〉B〉C, with significant difference （P〈0.05）. Pearson analysis showed peripheral blood NKT negatively correlated with the urine tubercle bacillus-DNA（ r = -0. 878, P（0. 0001）. Conclusions The quantity of NKT decreased and its function were suppressed in patients with nephrotuberculosis, and NKT related with the staging of nephrotuberculosis. Peripheral blood NKT negatively correlated with the urine tubercle bacillus-DNA.