摘要
The present study observed the effects of MCI-186 on the intracellular calcium ion concentration （[Ca2＋]i） and membrane fluidity of hippocampal neurons in Alzheimer＇s disease （AD） model rats to validate the neuroprotective effects of MCI-186. Compared with normal and sham-operated rats, the escape latency was obviously prolonged, spanning platform times were obviously reduced, [Ca2＋]i was increased, and microviscosity η value was increased in AD rats. MCI-186 at 0.5 mg/mL and 0.2 mg/mL obviously shortened escape latency, increased spanning platform times and decreased [Ca2＋]i and microviscosity η values in AD rats at 7 and 17 days after induction of the model. The differences in the above-mentioned indices between normal and MCI-186-treated rats were statistically significant. MCI-186 at 0.2 mg/mL yielded better curative effects than 0.5 mg/mL MCI-186. These findings suggest that MCI-186 improves learning and memory abilities in AD rats by decreasing [Ca2＋]i and increasing membrane fluidity of hippocampal neurons.
The present study observed the effects of MCI-186 on the intracellular calcium ion concentration （[Ca2＋]i） and membrane fluidity of hippocampal neurons in Alzheimer＇s disease （AD） model rats to validate the neuroprotective effects of MCI-186. Compared with normal and sham-operated rats, the escape latency was obviously prolonged, spanning platform times were obviously reduced, [Ca2＋]i was increased, and microviscosity η value was increased in AD rats. MCI-186 at 0.5 mg/mL and 0.2 mg/mL obviously shortened escape latency, increased spanning platform times and decreased [Ca2＋]i and microviscosity η values in AD rats at 7 and 17 days after induction of the model. The differences in the above-mentioned indices between normal and MCI-186-treated rats were statistically significant. MCI-186 at 0.2 mg/mL yielded better curative effects than 0.5 mg/mL MCI-186. These findings suggest that MCI-186 improves learning and memory abilities in AD rats by decreasing [Ca2＋]i and increasing membrane fluidity of hippocampal neurons.
基金
the Natural Science Foundation of Heilongjiang Province,No.D200807
Projects of Clinical Medicine in Science and Technology Development of Jiangsu University,No.JLY20080013
