去卵巢牙周炎模型大鼠牙槽骨吸收与补骨脂的干预

Effects of psoralea corylifolia on alveolar bone absorption in an ovariectimized rat model of periodontitis

背景:补骨脂在治疗骨质疏松症方面有明显效果。目的:观察补骨脂对去卵巢牙周炎大鼠牙槽骨代谢、牙槽骨密度及高度的影响。方法:将30只雌性Wistar大鼠随机等分为3组:去卵巢组和补骨脂组行双侧卵巢切除术,假手术组仅手术不切除卵巢,补骨脂组于卵巢切除第2天灌胃3g/kg补骨脂水剂,1次/d。去卵巢2周,所有大鼠采用结扎上颌磨牙的方法建立牙周炎模型。结果与结论:上颌磨牙结扎12周,与假手术组比较,去卵巢组大鼠血清雌二醇、钙离子浓度显著降低(P<0.05),碱性磷酸酶水平显著升高(P<0.05),X射线片及苏木精-伊红染色显示去卵巢组大鼠上颌骨骨密度及高度显著降低(P<0.05);与去卵巢组比较,补骨脂组大鼠血清钙离子浓度显著升高(P<0.05),碱性磷酸酶水平显著降低(P<0.05),上颌骨骨密度及高度显著增高(P<0.05),且与假手术组比较差异无显著性意义(P>0.05)。说明雌激素缺乏促进实验性牙周炎大鼠牙槽骨吸收,补骨脂可有效阻止雌激素降低导致的牙槽骨高度、骨密度降低。

BACKGROUND：Psoralea corylifolia exhibits obvious therapeutic effects on osteoporosis.OBJECTIVE：To investigate the effects of psoralea corylifolia on alveolar bone metabolism,alveolar bone mineral density and height in ovariectimized rats with periodontitis.METHODS：Thirty female Wister rats were randomly divided into three groups：ovariectomy,psoralea corylifolia and sham-surgery.Rats from the ovariectomy and psoralea corylifolia groups underwent bilateral ovariectomy,and rats from the sham surgery group did not receive ovariectomy.At 2 days after ovariectomy,rats from the psoralea corylifolia group were orally administered 3 g/kg psoralea corylifolia,once a day.At 2 weeks after ovariectomy,all rats were established into peridontitis models by ligation of maxillary molar.RESULTS AND CONCLUSION：At 12 weeks after ligation of maxillary molar,compared with sham surgery group,estradiol and calcium ion levels were significantly decreased（P 0.05）,alkaline phosphatase level was significantly increased（P 0.05） in the ovariectomy group.X-ray and hematoxylin-eosin staining showed that alveolar bone mineral density and height in the ovariectomy group was significantly decreased than that in the control group（P 0.05）.Compared with ovariectomy group,serum level of calcium ion was significantly increased（P 0.05） and alkaline phosphatase level was significantly decreased（P 0.05）,and alveolar bone mineral density and height were significantly increased in the psoralea corylifolia group.However,no significant difference was observed between psoralea corylifolia group and sham surgery group（P 0.05）.These findings suggest that estrogen-deficient state can promote alveolar bone absorption in rats with experimental periodontitis,and psoralea corylifolia can prevent the decrease in alveolar bone height and density resulting from estrogen deficiency.