京尼平苷对大鼠软骨细胞增殖的影响

Effect of geniposide on proliferation of rat chondrocytes in vitro

背景:京尼平苷能够促进韧带成纤维细胞的增殖和胶原的合成,促进组织的修复和韧带损伤的愈合。目的:观察京尼平苷对大鼠软骨细胞增殖的影响。方法:采用二次酶消化法获得大鼠关节软骨细胞进行传代培养,并取第4代细胞给予0,25,50,100mmol/L京尼平苷干预,利用MTT比色、流式细胞仪检测细胞周期等方法检测药物干预后第4代软骨细胞的增殖情况。结果与结论:体外培养的软骨细胞随着传代次数的增加,细胞形态由原代的多角形逐渐变为长梭形;且随着培养时间的延长及京尼平苷浓度的升高,软骨细胞增殖更为显著(P<0.01)。

BACKGROUND：Studies have shown that geniposide can ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen.OBJECTIVE：To investigate the effect of geniposide on the proliferation of rat chondrocytes in vitro.METHODS：The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in serial.The chondrocytes of passage 4 were interfered with 0,25,50,100 mmol/L geniposide.Chonddrocyte proliferation was detected using methyl thiazolyl tetrazolium and flow cytometry.RESULTS AND CONCLUSION：The phenotype of chondrocyte was changed from original polygonal shape to fusiform with the increased passage.The proliferation of chondrocytes in the 25,50,100 mmol/L geniposide groups was respectively higher than that in the control group（P 0.01）.Geniposide can stimulate the proliferation of chondrocytes.