流体切应力对成骨细胞Wnt/β-连锁蛋白信号转导通路的影响

Effects of fluid shear stress on Wnt/β-catenin signal transduction pathway in fibroblasts

背景:力学刺激对Wnt/β-连锁蛋白信号转导通路对成骨细胞的分化,增殖和凋亡有重要作用。Wnt/β-连锁蛋白信号转导通路的影响是近年来研究的热点。目的:探讨流体切应力对Wnt/β-连锁蛋白信号转导通路的影响在促进骨细胞骨形成和修复中的作用。方法:应用计算机检索CNKI期刊全文数据库和PubMed数据库(1990-01/2009-12)与流体切应力对成骨细胞Wnt/β-连锁蛋白信号转导通路影响有关的文章,纳入43篇符合标准的文献进行综述。结果与结论:力学负荷刺激Wnt/β-连锁蛋白信号转导通路可提高LRP5G171V鼠骨细胞骨形成敏感性,导致骨量增多,密度增大。体内和体外力学实验都支持Wnt/β-连锁蛋白信号转导通路是力学刺激的正常反应通路,Wnt/β-连锁蛋白信号转导通路在力学刺激作用下能提高成骨或骨细胞的成骨敏感性。

BACKGROUND：Mechanical stimulation exerts an important role in effects of Wnt/β-catenin signal transduction pathway on differentiation,proliferation and apoptosis of fibroblasts.OBJECTIVE：To explore the role of fluid shear stress in promoting bone formation and fracture healing influenced by Wnt/β-catenin signal transduction pathway.METHODS：A computer-based retrieval was performed in CNKI and PubMed database to search the manuscripts regarding fluid shear stress published between January 1990 and December 2009 RESULTS AND CONCLUSION：43 papers were included in the final analysis.Mechanically stimulating Wnt/β-catenin signal transduction pathway can increase the sensitivity to bone formation of LRP5 G171V mouse cells and increase bone mass and bone density.The in vivo and in vitro mechanical loading results support that Wnt/β-catenin signaling is a normal physiological response to load and that activation of the Wnt/β-catenin pathway enhances the sensitivity of osteoblasts/osteocytes to mechanical loading.