多聚赖氨酸对脂肪来源干细胞三维立体条件下生物学特性的影响

Poly-L-lysine bio-effects on the three-dimensional culture of rat adipose-derived stem cells

背景:多聚赖氨酸可促进软骨细胞、表皮细胞的黏附、生长及增殖。目的:观察多聚赖氨酸对脂肪来源干细胞三维立体培养下生物学活性的影响。方法:采用流式细胞仪检测昆明小鼠脂肪来源干细胞表面CD29、CD34、CD44、CD45的表达,将脂肪来源干细胞分别与经多聚赖氨酸表面修饰的珊瑚羟基磷灰石或空白珊瑚羟基磷灰石体外复合培养。结果与结论:与空白珊瑚羟基磷灰石组比较,经多聚赖氨酸修饰的珊瑚羟基磷灰石上黏附的脂肪来源干细胞较空白珊瑚羟基磷灰石组多,并分泌较多细胞基质,复合培养2,4,8d的A值较明显增高(P<0.05);但经多聚赖氨酸修饰的珊瑚羟基磷灰石上黏附的脂肪来源干细胞碱性磷酸酶活性值升高不明显(P>0.05)。说明多聚赖氨酸能促进脂肪来源干细胞在珊瑚羟基磷灰石表面的黏附、生长和增殖,不影响脂肪来源干细胞向成骨细胞转化。

BACKGROUND： Poly-L-lysine （PLL） can increase the adhesion, growth and proliferation of chondrocytes and epidermal cells. OBJECTIVE： To investigate the biological effects of PLL on three -dimensional cultured rat adipose-derived stem cells （ADSCs）. METHODS： The molecular markers （CD29, CD34, CD44 and CD45） of ADSCs were detected by flow cytometry, and then ADSCs were cultured on coralline hydroxyapatite （CHA） coated with PLL or not, respectively. RESULTS AND CONCLUSION： The CD29 and CD44 antigens could be detected, but the CD34 and CD45 were negative. The number of cells of PLL group was more than that of blank group, the PLL group’s determination value of MTT was increased significantly which was compared with those of the blank group （P 0.05） and the activity of alkaline phosphatase was not increased significantly （P 0.05）. The PLL can apparently increase the capacity of ADSCs adhesion, growth and proliferation, and cannot influence the osteogenic differentiation of ADSCs.