胶原-纳米羟基磷灰石复合支架的细胞相容性

Cellular compatibility of a nano-hydroxyapatite/collagen scaffold

背景:观察成骨细胞在生物材料上的形态、增殖和分化等项目,可评估生物支架材料的生物相容性。目的:观察复合支架材料纳米羟基磷灰石/胶原对成骨细胞增殖、分化的影响。方法:取新生24h内Wistar大鼠的颅盖骨,采用改良胶原酶消化法进行成骨细胞原代培养,取第3代细胞与纳米羟基磷灰石/胶原支架或普通羟基磷灰石材料体外复合培养。培养3,6,9d后,观察材料周边的细胞形态及支架材料对细胞分化、增殖的影响。结果与结论:纳米羟基磷灰石/胶原材料较普通的羟基磷灰石材料更有利于成骨细胞的黏附、生长、分化、增殖,证实其生物相容性更好,有望成为一种新型的骨组织工程支架材料。

BACKGROUND： Under the theory of bone-tissue engineering, the biocompatibility of biomaterial scaffolds is evaluated by observing the morphology, proliferation and differentiation of osteoblasts on the biomaterials. OBJECTIVE： To observe the effects of nano-hydroxyapatite/collagen （nHAC） scaffold on the proliferation and differentiation of osteoblasts. METHODS： The rat osteoblasts were obtained from the cranium of newborn Wistar rats within 24 hours, and primarily cultured using modified collagenase digestion. The cells at passage 3 were co-cultured with nHAC in vitro. At 3, 6 and 9 days of the culture, cell morphology was observed by inverted phase-contrast microscopy. In addition, MTT assay and alkaline phosphatase （ALP） activity test were used to observe the effects of the material on cell differentiation and proliferation. RESULTS AND CONCLUSION： Osteoblasts can adhere, grow, differentiate and proliferate better on nHAC than HA. The composite scaffold nHAC has a good compatibility, indicating that the material has a great potential for application in bone tissue engineering.