纤维蛋白胶复合重组人骨形态发生蛋白2微球对犬骨髓基质细胞增殖与分化的影响

Influence of recombinant human bone morphogenetic protein-2 loaded microspheres combined with fibrin glue on the proliferation and differentiation of canine bone marrow stromal cells

背景:前期实验证实聚乳酸-聚乙醇酸微球/纤维蛋白胶能作为重组人骨形态发生蛋白2的良好可注射性缓释载体。目的:观察可注射性骨形态发生蛋白缓释体系对犬骨髓基质细胞增殖与分化的影响。方法:采用复乳-溶剂挥发法制备重组人骨形态发生蛋白2/聚乳酸-聚乙醇酸共聚物载药微球,然后将微球与纤维蛋白胶复合制备出重组人骨形态发生蛋白2/聚乳酸-聚乙醇酸共聚物/纤维蛋白胶复合材料,采用细胞培养及组织化学等方法观察微球对犬骨髓基质细胞的增殖与分化的影响。结果与结论:重组人骨形态发生蛋白2/聚乳酸-聚乙醇酸共聚物/纤维蛋白胶微球对骨髓基质细胞的增殖无明显影响,但对细胞的分化功能有明显的促进作用。说明纤维蛋白胶复合重组人骨形态发生蛋白2微球能够提高骨髓基质细胞的体外成骨能力,可作为骨形态发生蛋白的良好载体。

BACKGROUND： It has been proved that poly （lactic-co-glycolic） acid （PLGA） microsphere/fibrin glue （FS） has satisfactory slow-release effect as recombinant human bone morphogenetic protein-2 （rhBMP-2） carrier. OBJECTIVE： To investigate the influence of injectable BMP release system by combining rhBMP-2/PLGA microsphere with FS on the proliferation and differentiation of canine bone marrow stromal cells （BMSCs）. METHODS： The rhBMP-2/PLGA microsphere was prepared using W/O/W solvent evaporation methods. rhBMP-2 loaded PLGA microsphere were incorporated in FS to establish injectable BMP release system. The influence of injectable BMP release system on BMSCs proliferation and differentiation were investigated by histochemistry method and cell culture. RESULTS AND CONCLUSION： Inhibition of BMSCs proliferation did not occur in response to in vitro culture with the rhBMP-2/PLGA microsphere/FS composite, but the differentiation of BMSCs was increased. rhBMP-2/PLGA microsphere/FS composite has satisfactory effect on improving in vitro osteogenic potential of BMSC as a good carrier of BMP.