自体嗅鞘细胞联合β-七叶皂甙钠修复大鼠脊髓全横断损伤

Transplantation of autologous olfactory ensheating cells combined with beta-aescinate sodium for repair of spinal cord transection in rats

背景:神经损伤时移植嗅鞘细胞能促进轴突再生,减少星形胶质细胞增生。目的:探讨自体嗅鞘细胞移植联合中药β-七叶皂甙钠对脊髓继发性损伤的保护作用。方法:制作SD大鼠脊髓损伤模型,随机分为对照组(注射生理盐水)、自体嗅鞘细胞移植组、自体嗅鞘细胞移植联合β-七叶皂甙钠治疗组。结果与结论:脊髓损伤后脊髓血管源性水肿、基质金属蛋白酶9表达上调,大鼠运动功能缺失,经自体嗅鞘细胞移植及自体嗅鞘细胞联合β-七叶皂甙钠治疗后脊髓水肿明显减轻、基质金属蛋白酶9表达下调,大鼠运动功能恢复,以自体嗅鞘细胞移植联合β-七叶皂甙钠治疗效果更明显。说明自体嗅鞘细胞移植联合β-七叶皂甙钠可更有效修复损伤脊髓,发挥保护作用。

BACKGROUND： Transplantation of olfactory ensheating cells （OECs） can promote axon regeneration and reduce the proliferation of astrocytes in spinal cord injury. OBJECTIVE： To explore the protective effects and mechanism of transplantation of autologous OECs combined with β-aescinate sodium in spinal cord injury rats. METHODS： Healthy Sprague-Dawley rats were divided into three groups： control, autologous OECs transplantation （OECs）, transplantation of autologous OECs combined with β-aescinate sodium （OECs ＋ β-aescinate sodium）. RESULTS AND CONCLUSION： Vasogenic spinal cord edema and matrix metalloproteinase 9 expression were increased, and rat motor function was lost. After transplantation of autologous OECs combined with β-aescinate sodium, spinal cord edema was obviously alleviated, MMP-9 expression was downregulated, rat motor function was recovered. These symptoms were obvious in the OECs ＋ β-aescinate sodium group. Results showed that transplantation of autologous OECs combined with β-aescinate sodium can effectively repair spinal cord injury and show protective effects.