微囊化甲状旁腺兔-鼠移植

Xenotransplantation of microencapsulated rabbit parathyroid tissue into rats

背景：微囊化移植技术已经普遍应用于各种实验,成为解决甲状旁腺移植免疫排斥反应的热点领域,但最佳移植部位及其移植失效机制仍是当前亟待解决的问题。目的：观察微囊化异种甲状旁腺组织移植对Wistar大鼠甲状旁腺功能低下的治疗作用,并探讨最佳移植部位及其失效机制。方法：制备甲状旁腺功能低下Wistar大鼠模型,建模4周后随机分为肾包囊组、胸锁乳突肌组,空囊组,肾包囊组、胸锁乳突肌组分别将微囊包裹兔甲状旁腺移入鼠肾包囊和胸锁乳突肌,空囊组仅于肾包囊移植空囊。移植后每隔2周取血测血钙,移植后第16周取移植物进行透射电镜检查。结果与结论：肾包囊组、胸锁乳突肌组16周内血钙水平差异无显著性意义（P〉0.05）,16~20周胸锁乳突肌组血钙呈下降趋势。胸锁乳突肌组微囊周围有大量纤维包裹。提示海藻酸-钡交联微囊可对甲状旁腺组织起到有效的保护作用,使甲状旁腺组织较长时间存活并发挥正常功能。肾包囊是较佳移植部位。

BACKGROUND： Microencapsulation has been widely used in various experimental studies. Microencapsulated parathyroid transplantation has become a hot issue, but the better transplantation site and the mechanism underlying failed transplantation are urgently to be solved. OBJECTIVE： To investigate the therapeutic effects of xenotransplantation of microencapsulated parathyroid tissue on treatment of hypoparathyroidism in Wistar rats and to determine the optimal transplantation site and the mechanism underlying failed transplantation. METHODS： Wistar rat models of hypoparathyroidism were developed. At 4 weeks after model establishment, rat models were randomly allocated to four groups： renal adipose capsules, chest sternocleidomastoid muscle, and empty microcapsule control groups. Microcapsuled parathyroid tissues were transplanted into renal adipose capsules and chest sternocleidomastoid muscle in renal adipose capsules group and chest sternocleidomastoid muscle group respectively. Empty microcapsules without parathyroid were transplanted into renal adipose capsules in empty microcapsule group. Total serum calcium was measured every other week. In the 16th week, transplant beds were retrieved and subjected to electron microscopic examination. RESULTS AND CONCLUSION： There was no significant difference in serum calcium level between renal adipose capsule group and chest sternocleidomastoid muscle group during 16 weeks （P 0.05）. The serum calcium level in the chest sternocleidomastoid muscle group decreased between 16-20 weeks. There were many fiber tissues surrounding the microencapsules in the chest sternocleidomastoid muscle group. Parathyroid tissues can be protected successfully from Aliginate-Ba capsules and then survive functionally for a long time. The renal adipose capsule is the better transplantation site than chest sternocleidomastoid muscle.