小鼠卵巢免疫反应产物内GATA-4的表达定位

Localization of GATA-4 in immune reaction product of mouse ovary

背景:特异性转录因子GATA-4家族对动物生殖细胞分化和生殖过程中多种激素的调节有非常重要的作用。目的:观察小鼠卵巢内GATA-4免疫反应产物的特征与分布。方法:选取健康昆明种6周龄ICR小鼠6只,取卵巢进行石蜡切片,应用免疫组织化学ABC法,DAB显色观察GATA-4在此时期卵巢中的表达情况。结果与结论:GATA-4免疫反应细胞在6周龄小鼠的卵巢中均有分布,主要在初级卵泡和次级卵泡的颗粒细胞,在卵泡膜细胞上有少量分布,黄体上未见分布。说明小鼠卵巢内存在GATA-4。

BACKGROUND： GATA-4 transcription factor plays a critical role in mammalian reproduction. OBJECTIVE： To explore the characteristics and distribution of GATA-4 in immune reaction product of the ovaries of female mouse. METHODS： Six 6-week old female mice were selected for this study. Paraffin sections of the ovaries were made and GATA-4 expression was observed by immunohistochemical ABC method and diaminobenzidine visualization. RESULTS AND CONCLUSION： Positive expression of GATA-4 was primarily found in granulosa cells and thecal cells of the mice, with less expression in theca cells, and no expression in corepus luteum. These findings suggest that GATA-4 exists in mouse ovaries.