高迁移率族蛋白B1在肾脏冷缺血再灌注损伤中的作用

Potential role of high mobility group box 1 protein in renal cord ischemia/reperfusion injury

背景:缺血再灌注损伤是临床器官移植不可避免的病理生理过程,冷缺血再灌注损伤具有研究肾移植更强的针对性。目的:观察高迁移率族蛋白B1在大鼠肾脏冷缺血再灌注损伤中的作用。方法:将SD大鼠随机分为假手术组、冷缺血再灌注组、丙酮酸乙酯(可显著抑制高迁移率族蛋白B1的合成与释放)治疗组。冷缺血再灌注组和丙酮酸乙酯治疗组制冷缺血再灌注模型前分别经阴茎背静脉注射林格液与丙酮酸乙酯,假手术组将腹腔打开后经阴茎背静脉注射林格液,45min后关闭腹腔。结果与结论:冷缺血再灌注组和丙酮酸乙酯治疗组各时间点肌酐、高迁移率族蛋白B1、肿瘤坏死因子α、核因子κB水平均显著高于假手术组(P<0.01),其中冷缺血再灌注组上述指标高于丙酮酸乙酯治疗组(P<0.01)。表明高迁移率族蛋白参与了肾移植冷缺血再灌注的病理过程,丙酮酸乙酯能够减轻肾脏冷缺血再灌注损伤。

BACKGROUND： Ischemia/reperfusion injury is the inevitable pathophysiological process in clinical organ transplantation. Cold ischemia/reperfusion injury has more targeted in organ transplantation process. OBJECTIVE： To investigate the potential role and intervention of high mobility group box-1 （HMGB1） protein in rats with renal cold ischemia/reperfusion injury. METHODS： SD rats were randomly divided into three groups： sham-operated group （n=20）, cold ischemia/reperfusion injury group （n=20）, and treatment group （n=20）. Rats in the cold ischemia/reperfusion injury group and treatment groups were respectively administered Ringer’s solution and EP via the penile vein before preparation of cold ischemia/reperfusion injury models. The sham-operated group rats were administered Ringer’s solution after opening the abdominal cavity, and 45 minutes later, the abdominal cavity was closed. RESULTS AND CONCLUSION： Compared with sham-operated group, creatinine, HMGB1 protein, tumor necrosis factor-α and nuclear factor-κB levels were significantly higher in the cold ischemia-reperfusion injury group and treatment group （P 0.01）. The above indices were significantly higher in the cold ischemia-reperfusion injury group than in the treatment group （P 0.01）. These findings suggest that the HMGBl plays an important role in the pathological processes of renal cold ischemia reperfusion injury, and EP can reduce renal cold ischemia-reperfusion injury.