维生素K3对腺样囊性癌细胞株的生长抑制作用及其与As_2O_3的联合效果

Effect of menadione on growth of human adenoid cystic carcinoma cell line and synergistic effects when combined with arsenic trioxide

目的:探讨维生素K3(VitK3)对腺样囊性癌细胞的凋亡诱导作用及其与As2O3的联合效果。方法:体外培养人腺样囊性癌细胞株(SACC-83),分别以VitK3、As2O3以及两者联合孵育细胞,采用四甲基偶氮唑蓝(MTT)法检测对腺样囊性癌细胞的抑制率,Annexin-V/Pl双染色流式细胞技术检测凋亡细胞的百分数,倒置显微镜及AO/EB染色观察细胞凋亡后的形态。采用SPSS 13.0软件包对数据进行方差分析和t检验。结果:体外实验表明,VitK3能抑制SACC-83细胞生长并呈剂量和时间依赖性,细胞死亡方式以凋亡为主。VitK3与As2O3联合应用时,具有协同作用。结论:VitK3能抑制腺样囊性癌细胞生长,并且与As2O3联合时有协同作用。

PURPOSE： To investigate the apoptosis—induced effect of menadione（VitK3）on human adenoid cystic carcinoma cell line,and the synergistic effect when it was combined with arsenic trioxide（As2O3）.METHODS： The adenoid cystic carcinoma cells treated with VitK3 or As2O3 or VitK3 and As2O3,respectively,were cultured in vitro.Cell viability was determined by MTT assay.The percentage of apoptotic cells was determined by flow cytometry following annexin V/Pl staining.The cellular morphology after apoptosis was observed by inverted microscope and AO/EB dye.A SPSS 13.0 software package was used for variance analysis and t test.RESULTS： In vitro VitK3 showed excellent cytotoxicity in a time and concentration-dependent manner on the adenoid cystic carcinoma cell lines.The mode of death induced by Vit K3 was predominantly apoptosis in vitro.The synergistic effect of VitK3 combined with As2O3 was also significant.CONCLUSION： VitK3 can inhibit the growth of adenoid cystic carcinoma cells.The combination of VitK3 with As2O3 has synergistic effect and can decrease the adverse reaction of As2O3 in treating cancer.