不同环境设置对局灶性脑梗死大鼠梗死灶周围神经丝蛋白、胶质纤维酸性蛋白表达的影响

Effect of different emvironmental interventions on neurofilament,glial fibrillary acidic protein expression in rats after unilateral local cerebral infarction

目的观察不同环境对局灶性脑梗死大鼠梗死灶周围神经丝蛋白(neurofilament,NF)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达的影响。方法 SD大鼠140只。将实验动物随机分为假手术对照组(10只)和大脑中动脉阻断(MCAO)手术组(130只),再将MCAO手术组随机分为独居组(30只)、社会交往组(40只)、探索学习组(30只)和丰富环境组(30只)。假手术对照组和各MCAO手术组分别于术后7、28天随机选取5只大鼠处死,采用免疫组织化学染色,观察大鼠梗死灶周围皮质NF、GFAP阳性表达。结果社会交往组、探索学习组和丰富环境组术后7、28天NF、GFAP阳性表达均明显优于独居组和假手术对照组(P<0.05或<0.01)。结论社会交往、探索学习和丰富环境均能促进梗死灶周围NF、GFAP的阳性表达。

Objective To observe effects of different environmental interventions on neurofilament（NF） and glial fibrillary acidic protein（GFAP） expression in rats after unilateral local cerebral infarction. Methods Sprague- Dawley rats were randomly divided into sham operation （n = 10） and middle cerebral artery occlusion （MCAO） operation groups. The rats in MCAO group were subjected to electric coagulation of MCA and then were assigned again randomly to individual living group （ n ~ 30）, social communication group （ n = 40）, learning group （ n = 30）, rich environment group（ n=30）. The rats were randomly sacrificed at different time points. The expressions of NF and GFAP in peri-ischemie cortex were examined by immunohistochemistry staining. Results After MCAO, the expression of NF and the number of GFAP labeled cells in social communication,learning and rich environment group were higher than those in sham and individual living group on day 7 and 28 after operation（ P 〈0.05 or 〈0.01）. Conclusion Social interaction,learning and enriched environment after MCAO could up-regulate the expression of NF and GFAP in the peri-isehemie regions.