初次自然流产与复发性自然流产绒毛组织细胞遗传学分析

Chromosomal karyotype in chorionic villi in patients with first spontaneous abortion or recurrent spontaneous abortion

目的:探讨绒毛细胞染色体核型异常与初次自然流产及复发性流产的关系。方法:根据104例早期自然流产孕妇的临床资料分为复发性自然流产组(57例)和初次自然流产组(47例)。清宫时取绒毛组织,按常规技术方法制备绒毛细胞染色体,G显带后进行核型分析。结果:复发性自然流产组染色体数目异常22例(39%),初次自然流产组染色体数目异常30例(67%)。复发性自然流产组绒毛细胞染色体核型异常率以及常染色体三体发生率均低于初次自然流产组(P均<0.05),两组X单体发生率比较差异无统计学意义(P>0.05)。结论:早期胚胎染色体数目异常是导致早期流产的主要原因。除细胞遗传学异常外,复发性自然流产的防治需积极寻找其他致病因素。

Objective： To investigate the correlation between abnormal karyotypes of fetal chorionic villus cells and first spontaneous abortion, recurrent spontaneous abortion. Methods： The subjects were divided into two groups： early recurrent abortion group （n = 57） and first early spontaneous abortion group （n = 47）. Fetal villus tissues were collected in uterine curettage for karyotyping by the conventional G-banding chromosome analysis. Resuits： Chromosome number abnormality was found in 22 cases （39.3%） and 30 cases （66.7%） in recurrent abortion group and first early spontaneous abortion group, respectively. Significant lower abnormal karyotype rate or triploid was observed in recurrent abortion group than that in first early spontaneous abortion group （42.9% vs 66.7% and 21.4% vs 57. 8% , P 〈 0. 05 ）. However, no significant difference was found in sexual chromosome abnormalities between the two groups （12. 5% vs 8.9%, P =0. 565）. Conclusion： The chromosome abnormality contributs significantly to early spontaneous abortion. However, other predisposing factors should be taken into account in early recurrent abortion.