杜仲内生真菌的分离鉴定及其对异甜菊醇的生物转化研究

Isolation and identification of endophytic fungi from Eucommia ulmoides and their biotransformation activity of isosteviol

目的:从植物杜仲的根、茎、叶中分离内生真菌并进行初步鉴定,并研究获得的杜仲内生真菌对异甜菊醇的生物转化活性。方法:采用形态学方法对分离菌株进行鉴定,并对其进行异甜菊醇生物转化活性筛选实验。结果:从健康杜仲的根、茎、叶中分离获得52株内生真菌,属于2纲,2目,4科,10属。其中有4株内生真菌对异甜菊醇具有生物转化活性,分别为交链孢霉Alternaria sp.EL3、瘤座霉Tubercularia sp.ER3、镰孢霉Fusarium sp.EL7及青霉Penicillium sp.ES2。结论:杜仲内生真菌多样性丰富,部分内生真菌对异甜菊醇具有生物转化活性,具有很好的研究和应用价值。

Objective： To isolate and identify some endophytic fungi from the root,stem and leaf of Eucommia ulmoides and to test their biotransformation activity of isosteviol.Methods： Endophytic fungi were morphologically identified and their biotransformation activity of isosteviol were screened.Results： 52 strains of endophytic fungi were isolated from Eucommia ulmoides,among which 4 strains presented biotransformation activity of isosteviol.Conclusion： The endophytic fungi of Eucommia ulmoides is diversiform and rich.They possess outstanding biotransformation activity of isosteviol and have excellent research and application value.