不同骨内植入材料对巨噬细胞活性的影响

Effect of Different Implant Materials on the Cells Viability of Macrophage

目的:研究不同骨植入材料对小鼠单核巨噬细胞RAW264.7活性的影响。方法:在钛(Ti)、医用不锈钢(SS)和聚甲基丙烯酸甲酯(PMMA)材料表面及细胞培养板(PSC)上培养小鼠巨噬细胞系RAW264.7,在不同时间用cck-8法测定细胞增殖情况,实时荧光定量PCR法测定单核细胞趋化蛋白-1(MCP-1)、转化生长因子-β1(TGF-β1)和肿瘤坏死因子-α(TNF-α)mRNA表达水平。结果:小鼠巨噬细胞系RAW264.7在各种材料上均能良好生长,无明显细胞毒性。Ti和PSC组细胞增殖速率明显高于SS和PMMA组。各组材料表面巨噬细胞MCP-1mRNA表达随时间减少,PMMA组表达较高。除钛组外,其余各组巨噬细胞TGF-β1mRNA表达水平随时间增加,12h和24h时钛组表达较高。TNF-αmRNA的表达水平随时间增加,Ti组和PSC组显著高于SS和PMMA组。结论:骨内植入材料表面的巨噬细胞增殖活性和基因表达受不同材料和接触时间的影响。

Objective： To observe the effect of different implant materials on the cells viability of macrophage RAW264.7.Methods： Macrophage RAW264.7 cells were seeded and cultured on pure titanium（Ti） disks,stainless steel（SS） disks,polymethyl methacrylate（PMMA） disks,and polystyrene cell-culture（PSC） for three days.Cell counting kit-8（CCK-8） assays were carried out to observe the cell proliferation on the different materials.Real-time PCR was used to measure the mRNA level of monocyte chemoattractant protein-1（MCP-1）,transforming growth factor-β 1（TGF-β1）,and tumor necrosis factor alpha（TNF-α）.Results： The proliferation rates of macrophage on Ti and PSC were significantly higher than that of SS and PMMA except at 12h.MCP-1 mRNA level of macrophages was decreased with time and was higher on PMMA.TGF-β1 mRNA levels of macrophages was increased with time except Ti.At 12h and 24h,TGF-β1 mRNA levels of macrophages was higher on Ti.TNF-α mRNA level of macrophages was increased on all materials and higher on Ti and PSC.Conclusion： The proliferation and gene expression of macrophage cultured on different materials is affected by materials and culture time.