核仁素和Ki-67在口腔鳞癌与癌前病变中的表达及意义

Expression and Significance of Nucleolin and Ki-67 in Squamous Cell Carcinoma of Oral Cavity and Precancerous Lesion Tissue

目的:探讨核仁素和ki-67在口腔正常黏膜(Normal oral mucosa,NOM)、口腔黏膜白斑(Leukoplakia,LK)和口腔鳞状细胞癌组织(Oral Squamous Cell Carcinoma,OSCC)中表达的变化,研究核仁素在口腔癌发病中的表达及其临床意义。方法:采用免疫组织化学PV法检测10例NOM、34例LK伴异常增生上皮和47例OSCC组织中核仁素及Ki-67的表达。采用SPSS17.0进行统计分析。结果:光镜下核仁素和Ki-67的阳性部位为细胞核,但核仁素在OSCC的部分细胞的胞浆和/或胞膜亦有阳性表达。核仁素和Ki-67在NOM,LK,OSCC的表达强度依次增高,差异有显著性(P<0.05)。核仁素和Ki-67随着LK和OSCC的病理分级升高表达强度逐渐升高;核仁素和Ki-67在OSCC的分化(高分化比中低分化)差异均有显著性(P<0.05)。其中,核仁素在NOM和OSCC,NOM和LK,OSCC浸润肌层差异均有显著性(P<0.05)。Ki-67在NOM和OSCC,LK与OSCC差异有显著性(P<0.05)。核仁素和Ki-67在淋巴结转移,性别,年龄,民族,吸烟,饮酒表达差异无显著性。经Spearman相关分析,核仁素和Ki-67在NOM,LK和OSCC病理分级表达的阳性率中分布呈正相关(r=0.90,P<0.05)。结论:核仁素可能参与OSCC的发生、发展,且与其亚细胞定位的变化有一定关系,在OSCC发生浸润方面有较高的敏感性。其与Ki-67一样可以作为细胞增殖的一个指标。

Objective： To study the expression and clinicopathology significance of nucleolin and Ki-67 in normal oral mucosa（NOM）,leukoplakia（LK） and oral squamous cell carcinoma（OSCC） by tissue array analysis.Methods： The expression of nucleolin and Ki-67 protein was examined by the immunohistochemical technique（PV） in 10 NOM,34 LP,and 47 OSCC samples,and the experimental data were statistically analyzed by SPSS 17.0 software.Results： The labels of nucleolin and Ki-67 were located in nucleolus,but Nucleolin also labeled in cytosols and/or membranes of some other tumors cells examined by optical microscope.There were significant differences of nucleolin and Ki-67 between NOM,LK and OSCC（P0.05）.The expression of Nucleolin and Ki-67 were higher in LK and OSCC with poorer grade of histologic differentiation,and dffferent significantly in histologic grade（well vs moderately/poorly）（P0.05）.The expression of nucleolin were observably different between NOM and OSCC,NOM and LK,and muscle invasin.And significant different expression of Ki-67 was between NOM and OSCC,LK and OSCC（P0.05）.There were no differences between those with regional lymph node metastasis,sexes,ages,ethnicitices,drinking or smoking.The expression of nucleolin and Ki-67 was correlated in positive rate of histologic differentiation grade of NOM,LK and OSCC（P0.05）.Conclusion： Expression of nucleolin protein may play an important role in progression of OSCC,which was associated with subcellular location.And it was more sensitive in OSCC muscle invasion.Nucleolin has the protential of being a new molecular target,as Ki-67,of cell proliferation.