羊蹄蒽醌类成分的质控方法研究

Study on the Quality Control of Anthraquinones in Rumex japonicus Houtt.

目的:建立羊蹄药材中蒽醌类成分鉴别和含量测定方法,为羊蹄药材质量评价提供新依据。方法:采用薄层色谱法对羊蹄药材进行定性鉴别,超高速液相色谱法(UPLC)建立羊蹄药材中大黄素、大黄酚、大黄素甲醚等成分的含量测定方法。结果:羊蹄药材中大黄素、大黄酚、大黄素甲醚等成分的薄层鉴别特征明显,UPLC法能快速地同时测定羊蹄中大黄素、大黄酚和大黄素甲醚的含量。结论:该方法具有良好的专属性和重现性,能准确、稳定、快速地对羊蹄药材进行鉴别和定量测定,可作为羊蹄药材中蒽醌类成分的质量控制方法。

Objective： To establish the new methods that can identify and assay the anthraquinones of Rumex japonicus Houtt. while be used to evaluate the quality of the Rurnex japonicus Houtt.. Methods： Thin layer chromatography（ TLC ）was used for the identification and UPLC was applied to assay the contents of emodin, chrysophanol and physcion of Rumex japonicus Houtt.. Results： The characteristic of TLC was distinct. It showed that the UPLC can fast assay the contents of emodin, chrysophanol and physcion at one time. Conclusion： These methods can be used in qualitative identification and quantitative assay of anthraquinones of Rumex japonicus Houtt. with good specificity, reproducibility and stability.