右美托咪啶对胃穿孔修补术大鼠血流动力学及苏醒时间的影响

目的探讨右美托咪啶对胃穿孔修补术大鼠血流动力学及恢复翻正反射时间的影响。方法选择右侧颈动脉置管成功的雄性SD大鼠20只,随机分成实验组(D组)和对照组(C组),每组10只。大鼠平均动脉压(MAP)监测建立后,实验组经腹腔注射右美托嘧啶30μg/kg(浓度4μg/ml),对照组注射同容量的生理盐水,腹腔注药15min后实施胃穿孔修补术。全程记录大鼠腹腔注药前(基础值)、注药后15min、以及整个手术过程的平均动脉压(MAP)变化,记录大鼠恢复翻正反射的时间。结果与C组比较,D组大鼠腹腔注药后5min和10minMAP的差异无统计学意义(P>0.05),注药后15minMAP下降,D组为(60.58±13.13)mmHg,C组为(81.82±18.75)mmHg,差异有统计学意义(P>0.05)。两组大鼠翻正反射恢复的时间,D组为(200.38±40.82)min,C组为(171.50±33.10)min,差异无统计学意义(P>0.05)。结论大鼠腹腔注射右美托嘧啶(30μg/kg)15min后MAP开始降低,其对大鼠恢复翻正反射的时间无影响。

Objective To investigate the impact of dexmedetomidine on the hemodynamics of gastric perforation repair and recovery time of righting reflex. Methods 20 male SD rats whose right carotid artery catheter were successful were randomly divided into experimental group （D group ） and control group （C group）, n = 10. After the establish of rats mean arterial pressure（MAP） monitoring, the experimental group were intraperitoneal injected with dexmedetomidine 30 ug/kg （concentration 4 ug/ml ）, and the control group was injected with the same volume of saline. Further, the gastric perforation repair were implemented after 15 min. Rats were monitored throughout before injection （ baseline）, after injection, the MAP and the entire surgical process changes, and rats were recorded recovery time of righting reflex. Results Compared with group C, D rats after intraperitoneal injection, 5 min and 10 rain MAP difference was notstatistieally significant （P 〉 0. 05 ）, decreased after injection 15 min MAP, D group, （60. 58 ± 13.13 ） ram Hg, C group was （ 81.82 ±18.75）mm Hg, the difference was statistically significant （P 〉0.05）. Two groups of rats after the recovery time of righting reflex, D group was （200. 38 ±40. 82） min, C group was （ 171.50 ±33.10） min, the difference was not statistically significant （P 〉 0. 05 ）. Conclusion Intraperitoneal injection of dexmedetomidine （30 ug/ kg） 15 min, MAP began to decrease, the recovery of righting reflex in rats had no effect on the time.