摘要
建立一种肉及其制品中莱克多巴胺的酶联免疫分析方法。对莱克多巴胺残留量进行选择性(交叉反应)、特异性与检测限以及回收率和精密度测试。结果显示,选择酶联免疫法来检测样品中的莱克多巴胺准确性较好,其对莱克多巴胺结构类似物的交叉反应率都小于1%,出口猪肉罐头和牛肉中的检测限(LOD)分别为(0.28±0.30)μg/kg和(0.26±0.21)μg/kg,样品平均回收率在82.9%～91.1%之间,相对标准偏差为4.3%～8.7%,经高效液相色谱(HPLC)确证假阳性率小于等于2.0%,表明酶联免疫分析定量法可快速、准确实现对食品中莱克多巴胺残留量的快速筛选。
This paper describes an enzyme-linked immunosorbent assay （ELISA） to determine ractopamine in meat and meat products. The proposed method was highly accurate in determining ractopamine and showed a cross-reactivity of less than 1% with all ractopamine analogues investigated. The limits of detection （LODs） for exported canned pork and exported beef were （0.28±0.30） μg/kg and （0.26±0.21） μg/kg, respectively, with an average spike recovery of 82.9%-91.1% （n = 15） and a relative standard deviation of 4.3%-8.7%. Using high performance liquid chromatography （HPLC）, the false posiitve rate of this method was confirmed as less than 2.0%, suggesting that it can enable accurate and rapid determination of ractopamine residues in foods.
出处
《肉类研究》
2011年第10期26-28,共3页
Meat Research
基金
安徽出入境检验检疫行业标准项目(2009B165)
