摘要
采用高效液相色谱-荧光法(HPLC-FLD)检测猪肉中两种氟喹诺酮类药物。猪肉样品经无水硫酸钠脱水后用1%醋酸+乙腈提取,提取液用正己烷饱和的乙腈净化,用流动相(乙腈、0.01mol/L磷酸二氢钠(磷酸调pH3)体积比10:90)定容。通过对猪肉样品中氟喹诺酮类药物的检测表明该方法具有准确、快速、灵敏的特点。
An HPLC with FLD （fluorescence detector） method was established for the determination of two kinds of fluoroquinolones in pork. Pork samples were extracted with 1% acetic acid/acetonitrile, followed by water removal by anhydrous sodium sulfate and clean-up by acetonitrile saturated n-hexane. The mobile phase was composed of acetonitrile and 0.01 mol/L sodium phosphate （pH 3, adjusted with phosphorous acid） at a ratio of 10：90 （V/V）. The analytes were separated on a ZORBAX-SB-CIs column and detected by a fluorescence detector. The established method proved accurate, rapidy and sensitive.
出处
《肉类研究》
2011年第10期29-31,共3页
Meat Research
基金
山东出入境检验检疫局科技项目(SK201172)
关键词
高效液相色谱法
氟喹诺酮类药物
残留分析
猪肉
high performance liquid chromatography （HPLC）
fluoroquinolone
residue analysis
pork
