陕西岚皋县魔芋软腐病原菌的分离与鉴定

Isolation and identification of Amorphophallus bacterial soft rot in Langao County,Shaanxi Province

【目的】确定导致陕西岚皋县魔芋(Amorphophallus)软腐病的主要病原菌类型。【方法】从陕西省岚皋县魔芋患病组织中分离、纯化致病菌,通过培养细菌的形态特征、致病性及分子生物学分析,确定病原菌的类型。【结果】从魔芋软腐病病植体中共分离得到27株菌,其中9株菌(M1～M9)可使健康植株致病,菌株M8致病性最强。通过生物学特性观察,确定M8果胶杆菌是魔芋致病的主要病原菌。其16SrDNA基因序列与胡萝卜软腐果胶杆菌胡萝卜软腐亚种(Pectobacterium carotovorum PC1)菌株关系最近,相似性为98.64%。【结论】导致岚皋县魔芋软腐病的病原菌是胡萝卜软腐果胶杆菌胡萝卜软腐亚种。

【Objective】 The research identified the pathogenic bacteria of Amorphophallus that suffered from soft rot disease.【Method】 The rot-causing bacteria isolated from amorphophallus in Langao county,Shaanxi Province were identified by 16S rDNA sequence analysis,pathogenicity and morphological characters.【Result】 27 strains were obtained,9 of which were rot-causing bacteria,strain M8 had a high capacity to cause soft rot disease.The investigation of the biological characteristics indicated that they had the same characteristics with Pectobacterium sp.16S rDNA sequence analysis of strains showed that they belonged to Pectobacterium sp.and were very closely related to P.carotovorum PC1（98.64%）.【Conclusion】 Amorphophallus bacterial soft rot in Langao County was determined as P.carotovora subsp.carotovora,Pcc.