北京地区成人雌马酚代谢表型与血清性激素关系研究

Equol-producing phenotype and in relation to serum sex hormones among healthy adults in Beijing

目的确定北京地区成人雌马酚代谢表型与血清性激素的关系。方法应用现况调查方法,调查北京市区180名20～72岁志愿者生活方式及膳食营养素摄入量;采用高效液相色谱法(HPLC)分析负荷大豆异黄酮(Iso)前后尿中雌马酚等大豆异黄酮24h排泄量,分析雌马酚代谢表型与血清性激素的关系。结果男性血清总睾酮浓度,女性血清雌二醇和孕酮浓度,在产雌马酚者与非产生者间差异无显著性(P>0.05)。在男性产雌马酚者中,日常膳食情况下的尿液总大豆异黄酮、大豆素、雌马酚、邻脱甲基安哥拉柴檀素、黄豆黄素和双氢黄豆黄素24h排泄量与血清总睾酮浓度呈负相关(r=-0.29～-0.36,P<0.05);而女性日常膳食情况下的尿液大豆异黄酮代谢产物24h排泄量与血清雌二醇和孕激素相关性无统计学意义(P>0.05),且与雌马酚代谢表型无关。结论日常膳食Iso暴露与男性血清总睾酮水平有关。

Objective To evaluate relations between equol-producing phenotype and serum sex hormones among adults in Beijing.Methods 90 male and 90 female adults participated in a cross-sectional study and provided twice 24h urine samples on a regular diet and after 3-d soy isoflavone challenge respectively.A health and demographics questionnaire,and 2 days food record were completed before the urine collection.Isoflavones and their metabolites in urine were measured to determine equol phenotype by HPLC.Results The serum total testosterone level of the male and the serum estradiol and progesterone levels among the female participants were no significant differences between the equol producers and non-producers（P0.05）.There were negative correlations between urinary total isoflavone,daidzein,equol,O-desmethylangolensinl,glysitein,dihydroglysitein levels and serum total testosterone concentration among the male equol producers on a regular diet（r =-0.29～-0.36,P0.05）.There were no significant correlations between urinary isoflavonoid excretion and serum estradiol and progesterone concentration among the female participants on a regular diet（P0.05）,regardless of equol phenotype.Conclusion The result suggests that exposure of isoflavone is correlated with the testosterone in healthy men under the usually lifestyle,and may be related to equol phenotype.