青海藏族人群ADH3、ALDH2基因多态性及其与饮酒行为的关系

Genetic Polymorphism of ADH3 and ALDH2 among Tibetan population in Qinghai and its relationship with drinking behavior

目的了解青海藏族男性乙醇脱氢酶3(ADH3)和乙醛脱氢酶2(ALDH2)基因多态性分布及其与饮酒行为的关系。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法对ADH3和ALDH2基因型进行检测;采用回顾性问卷调查研究对象的饮酒行为。结果等位基因ADH3*2和ALDH2*2在藏族人群中的比例分别为7.79%和22.21%;不饮酒组中等位基因ALDH2*2、ADH3*1频率高于饮酒者;危险饮酒组中等位基因ALDH2*2、ADH3*1频率低于安全饮酒者。结论 ADH3、ALDH2基因与青海藏族男性人群饮酒行为有关。

Objective To investigate the genetic polymorphism of ADH3 and ALDH2 among Tibetan population in Qinghai and its relationship with drinking behavior.Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） technique was used to detect the genotype of ADH3 and ALDH2.Drinking behavior was obtained by questionnaire.Results The frequency of ADH3 ＊ 2 and ALDH2 ＊ 2 alleles in Tibetan population was 7.79% and 22.21% respectively,the frequency of ALDH2 ＊ 2 and ADH3 ＊ 1 alleles in non-drinkers was higher than that in drinkers,and the frequency of ALDH2 ＊ 2 and ADH3 ＊ 1 alleles in risk drinkers was lower than that in safe drinkers.Conclusion ADH3 and ALDH2 are associated with drinking behavior in male Tibetan population.