天龙抗肿瘤小分子肽纯化工艺

Purification Technology of Anti-tumor Small Peptide from Gecko

目的:纯化天龙酶解液,制备小分子肽。方法:用石蜡萃取法除油脂,用Sephadex G-15除氨基酸和盐。结果:天龙酶解液中加入药材质量分数40%的石蜡,加热使完全融化,搅拌,冷藏(6℃)静置4 h,弃去石蜡,水液以每2 mL加于Sephadex G-15柱(1.6 cm×64 cm)上,用去离子水2 mL.min-1洗脱,收集第60～84 mL洗脱液。结论:所筛选的纯化工艺简便可行。

Objective：To purify enzymolysis liquid of Gecko,prepared small peptide.Method：Paraffin extraction was used to remove gease,and Sephadex G-15 was adopted to except AA and salts.Result：Added 40% paraffin into enzymolysis liquid of Gecko,melted it,stirred,stilled 4 h at 6 ℃,discarded paraffin,the remaining water solution was added onto Sephadex G-15（1.6 cm×64 cm） every 2 mL,eluted with deionized water by 2 mL·min-1,collected eluate after 60 mL and stopped at 84 mL.Conclusion：The selected purification technology was simple and feasible.