大孔树脂纯化祖师麻中香豆素类成分的工艺优选

Purification Technology of Coumarin from Cortex Daphnes by Macroporous Resin

目的:研究大孔树脂纯化祖师麻中香豆素类成分的工艺。方法:以祖师麻甲素、总香豆素为指标,考察了3种大孔树脂的吸附效果并系统研究大孔树脂对祖师麻中香豆素类成分的吸附性能和洗脱参数。结果:D-101大孔树脂对祖师麻甲素、总香豆素有较好的纯化性能,最佳上样工艺是药液质量浓度生药50 g.L-1,上柱流速2 BV.h-1,pH 3.0。上样后先用4 BV水洗脱,继以5 BV50%乙醇洗脱,洗脱液流速2 BV.h-1。结论:确定的大孔树脂纯化祖师麻中的香豆素类成分的工艺是合理的。

Objective：To establish purification technology of coumarin from Cortex Daphnes.Method： Investigated adsorption capacity of 3 kinds of macroporous resin with daphnetin and coumarins as indexes,and adsorption characteristics and elutive ratio of coumarin from Cortex Daphnes were studied systematically.Result： D-101 resin had a better purification performance to daphnetin and coumarins.Optimum conditions were as follows：concentration of durg 50 g·L-1 in terms of raw material,adsorption speed was 2 BV·h-1,pH of sample for loading 3.0.After eluted with 4 BV of distilled water,5 BV 50% ethanol was used with desorption speed was 2 BV·h-1.Conclusion： This process of applying macroporous resin to purify coumarin in Cortex Daphnes was reasonable and feasible.